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fixable viability markers ghost dye red 780  (Cytek Biosciences)


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    Structured Review

    Cytek Biosciences fixable viability markers ghost dye red 780
    Fixable Viability Markers Ghost Dye Red 780, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 508 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ghost+dye+red+780+viability+dye/Ghost+Dye+Red+780/pm41813793-143-5-15
    Average 96 stars, based on 508 article reviews
    fixable viability markers ghost dye red 780 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Staining:

    Article Title: GDF3 promotes adipose tissue macrophage-mediated inflammation via altered chromatin accessibility during aging.
    Article Snippet: Aging is characterized by amplified inflammation, including proinflammatory macrophages and increased susceptibility to endotoxemia.. Here we uncover a mechanism by which macrophages maintain their inflammatory phenotype through autocrine GDF3–SMAD2/3 signaling, which ultimately exacerbates endotoxemia.. We show that inflammatory adipose tissue macrophages display an age-dependent increase in GDF3, a TGFβ-family cytokine.

    Article Title: The STORE.2 model of the T cell proliferative phase considering c-Myc
    Article Snippet: Cells were washed with FACS buffer [1× PBS, 0.2% bovine serum antigen (Gemini, 700-100P), and 1 mM EDTA (Gibco, 15575-038)] and incubated in Fc block [99.5% FACS buffer, 0.5% normal rat immunoglobulin G (Invitrogen, 10700), and 2.4G2 (1 μg/ml; Bio X Cell, BE0307)] at 4°C for 10 min before staining. .. If cells were stained for cell death using LIVE/DEAD staining, then LIVE/DEAD Fixable Aqua Dead Cell marker (Invitrogen, L34957), Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences, 13-0870-T100), or Ghost Dye Red 780 Viability Dye (Tonbo Biosciences, 13-0865-T100) was included during incubation with Fc block. ..

    Article Title: An Il12 mRNA-LNP adjuvant enhances mRNA vaccine-induced CD8 T cell responses.
    Article Snippet: Optimizing vaccine design to induce CD8 T cell responses has been challenging, but lipid nanoparticle (LNP)– encapsulated mRNA vaccines effectively generate CD8 T cell memory.. Interleukin12 (IL12) supports CD8 T cell expansion and acquisition of effector function, but the role of IL12 in the generation of CD8 T responses to mRNA vaccination is unclear.. Here, we determine that endogenous IL12 is not required for CD8 T cell responses to mRNALNP vaccination.

    Article Title: GDF3 promotes adipose tissue macrophage-mediated inflammation via altered chromatin accessibility during aging
    Article Snippet: The templates for single guide RNA (sgRNA) synthesis were generated by PCR from a pX330 template; sgRNAs were transcribed in vitro and purified (Megashortscript, MegaClear; Thermo Fisher). .. For live–dead staining, cells were incubated with Ghost Dye Red 780 Viability Dye (Tonbo, 13-0865-T100) for 20 min on ice, in the dark. .. High-performing sgRNAs were selected, and repair template oligonucleotides (single-stranded oligodeoxynucleotide) incorporating loxP sites targeted to the Cas9 cleavage sites were synthesized by Integrated DNA Technologies.

    Incubation:

    Article Title: GDF3 promotes adipose tissue macrophage-mediated inflammation via altered chromatin accessibility during aging.
    Article Snippet: Aging is characterized by amplified inflammation, including proinflammatory macrophages and increased susceptibility to endotoxemia.. Here we uncover a mechanism by which macrophages maintain their inflammatory phenotype through autocrine GDF3–SMAD2/3 signaling, which ultimately exacerbates endotoxemia.. We show that inflammatory adipose tissue macrophages display an age-dependent increase in GDF3, a TGFβ-family cytokine.

    Article Title: The STORE.2 model of the T cell proliferative phase considering c-Myc
    Article Snippet: Cells were washed with FACS buffer [1× PBS, 0.2% bovine serum antigen (Gemini, 700-100P), and 1 mM EDTA (Gibco, 15575-038)] and incubated in Fc block [99.5% FACS buffer, 0.5% normal rat immunoglobulin G (Invitrogen, 10700), and 2.4G2 (1 μg/ml; Bio X Cell, BE0307)] at 4°C for 10 min before staining. .. If cells were stained for cell death using LIVE/DEAD staining, then LIVE/DEAD Fixable Aqua Dead Cell marker (Invitrogen, L34957), Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences, 13-0870-T100), or Ghost Dye Red 780 Viability Dye (Tonbo Biosciences, 13-0865-T100) was included during incubation with Fc block. ..

    Article Title: GDF3 promotes adipose tissue macrophage-mediated inflammation via altered chromatin accessibility during aging
    Article Snippet: The templates for single guide RNA (sgRNA) synthesis were generated by PCR from a pX330 template; sgRNAs were transcribed in vitro and purified (Megashortscript, MegaClear; Thermo Fisher). .. For live–dead staining, cells were incubated with Ghost Dye Red 780 Viability Dye (Tonbo, 13-0865-T100) for 20 min on ice, in the dark. .. High-performing sgRNAs were selected, and repair template oligonucleotides (single-stranded oligodeoxynucleotide) incorporating loxP sites targeted to the Cas9 cleavage sites were synthesized by Integrated DNA Technologies.

    IF-cells:

    Article Title: The STORE.2 model of the T cell proliferative phase considering c-Myc
    Article Snippet: Cells were washed with FACS buffer [1× PBS, 0.2% bovine serum antigen (Gemini, 700-100P), and 1 mM EDTA (Gibco, 15575-038)] and incubated in Fc block [99.5% FACS buffer, 0.5% normal rat immunoglobulin G (Invitrogen, 10700), and 2.4G2 (1 μg/ml; Bio X Cell, BE0307)] at 4°C for 10 min before staining. .. If cells were stained for cell death using LIVE/DEAD staining, then LIVE/DEAD Fixable Aqua Dead Cell marker (Invitrogen, L34957), Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences, 13-0870-T100), or Ghost Dye Red 780 Viability Dye (Tonbo Biosciences, 13-0865-T100) was included during incubation with Fc block. ..

    Marker:

    Article Title: The STORE.2 model of the T cell proliferative phase considering c-Myc
    Article Snippet: Cells were washed with FACS buffer [1× PBS, 0.2% bovine serum antigen (Gemini, 700-100P), and 1 mM EDTA (Gibco, 15575-038)] and incubated in Fc block [99.5% FACS buffer, 0.5% normal rat immunoglobulin G (Invitrogen, 10700), and 2.4G2 (1 μg/ml; Bio X Cell, BE0307)] at 4°C for 10 min before staining. .. If cells were stained for cell death using LIVE/DEAD staining, then LIVE/DEAD Fixable Aqua Dead Cell marker (Invitrogen, L34957), Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences, 13-0870-T100), or Ghost Dye Red 780 Viability Dye (Tonbo Biosciences, 13-0865-T100) was included during incubation with Fc block. ..

    Blocking Assay:

    Article Title: The STORE.2 model of the T cell proliferative phase considering c-Myc
    Article Snippet: Cells were washed with FACS buffer [1× PBS, 0.2% bovine serum antigen (Gemini, 700-100P), and 1 mM EDTA (Gibco, 15575-038)] and incubated in Fc block [99.5% FACS buffer, 0.5% normal rat immunoglobulin G (Invitrogen, 10700), and 2.4G2 (1 μg/ml; Bio X Cell, BE0307)] at 4°C for 10 min before staining. .. If cells were stained for cell death using LIVE/DEAD staining, then LIVE/DEAD Fixable Aqua Dead Cell marker (Invitrogen, L34957), Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences, 13-0870-T100), or Ghost Dye Red 780 Viability Dye (Tonbo Biosciences, 13-0865-T100) was included during incubation with Fc block. ..

    other:

    Article Title: Neddylation Targets and Stabilizes NLRP3 to Augment Inflammasome‐Mediated Colitis and Mood Disorder
    Article Snippet: Cells were then incubated with fluorescence‐conjugated antibodies PerCP‐CD45 (Cat# 103130, BioLegend), BV510‐CD11b (Cat# 101263, BioLegend), and PE‐F4/80 (Cat# 111604, BioLegend) for 30 min on ice.

    Article Title: TCF1 lo CD8 T cells proliferate and persist autonomously in tumors
    Article Snippet: 4′,6-diamidino-2-phenylindole (DAPI), BioLegend, Cat# 422801, Cas-No: 47165-04-8; Ghost Dye Violet 450 Viability Dye, Tonbo/Cytek, Cat# 13-0863; Ghost Dye Red 710 Viability Dye, Tonbo/Cytek, Cat# 13-0871; Ghost Dye Red 780 Viability Dye,Tonbo/Cytek, Cat# 13-0865.



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